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Hematological and immunological development from birth to six months of age in Holstein calves Arq. Bras. Med. Vet. Zootec.
Baccili,C.C.; Oliveira,S.M.F.N. de; Costa,J.F.R; Massoco,C.O.; Pozzi,C.R.; Gomes,V..
ABSTRACT The hematological and immunological development of calves from birth to 6 months of age was performed by hemogram and cellular phenotype. Ten male Holstein calves were assessed in 13 moments: before colostrum intake (D0), every 2 days until the 10th day of life (D2 to D10), at the 15th day after birth (D15), and then monthly up to 6 months (D180). Calves presented hemoconcentration on day (D) 0 and showed a gradual decrease in hematimetric rates until D180. The inversion of the neutrophilic for lymphocytic profile was observed on D4. The percentage of CD3+ cells on D10 was higher than D30 up to D180. The number of CD4+ and CD8+ cells did not change between time points. The number of CD21+ lymphocytes was significantly higher at early time points...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Immune system; Leukogram; Immunophenotyping; Flow cytometry.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601823
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Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects BJMBR
Malbouisson,L.M.S.; Szeles,T.F.; Barbalho,L.; Massoco,C.O.; Carmona,M.J.C.; Carvalho,C.R.R.; Pelosi,P.; Auler Jr.,J.O.C..
Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 ± 1.2 years, BMI: 23.8 ± 3.6 kg/m²). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH2O steps up to 20 cmH2O every 5 breaths. At CPAP 20 cmH2O, an inspiratory pressure of 20 cmH2O above CPAP was applied, reaching an airway pressure of 40 cmH2O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lung hyperinflation; Ventilator-induced lung injury; Inflammation; Cytokines.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200011
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Técnica de citometria de fluxo para avaliação da produção de espécies reativas de oxigênio pelas células do líquido sinovial de eqüinos Arq. Bras. Med. Vet. Zootec.
Massoco,C.O.; Carmona,L.; Baccarin,R.Y.A..
Using the cytometry flow analysis and adapting the method proposed by Hasui et al. (1985), it was possible to quantify the reactive oxygen species (ROE) produced by distinct cell populations of the synovial fluid from rigid and inflamed joints in horses, and to identify an increase in the polymorphonuclear cells population densities in the inflamed joints. These data strengthen the premise that cytometry flow techniques can contribute for future researches on the articular cartilage degeneration in horses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eqüino; Citometria de fluxo; Líquido sinovial; Explosão oxidativa.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400032
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The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs Arq. Bras. Med. Vet. Zootec.
Angrimani,D.S.R.; Nichi,M.; Brito,M.M.; Kawai,G.K.V.; Rui,B.R.; Losano,J.D.A.; Vieira,N.M.G.; Francischini,M.C.P.; Cruz,D.S.G.; Queiroz-Hazarbassanov,N.; Massoco,C.O.; Vannucchi,C.I..
ABSTRACT The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Cryopreservation; Antioxidant; Canine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200419
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